Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA‑4327 regulates TGF‑β1 stimulation of matrix metalloproteinase‑13 expression via CREB‑binding protein‑mediated Runx2 acetylation in human osteoblasts

doi: 10.3892/etm.2024.12770

Figure Lengend Snippet: Knockdown of CBP decreased TGF-β1-stimulated MMP-13 expression in human osteoblasts. Scrambled siRNA or CBP siRNA (30 nM) was transiently transfected into MG-63 cells for 24 h. Cells were treated with 5 ng/ml TGF-β1 or left untreated for 8 h. Western blot analysis of the whole-cell lysates was performed using antibodies against CBP, MMP-13, or α-Tubulin. α-Tubulin expression was used for normalization. CBP, CREB-binding protein; TGF-β1, transforming growth factor-β1; MMP-13, matrix metalloproteinase-13; si, small interfering.

Article Snippet: Scrambled control siRNA (cat. no. Sc-37007) and CBP siRNA (cat. no. Sc-29244) were purchased from Santa Cruz Biotechnology, Inc. miR-4327 mimic (GeneGlobe ID: YM00470747) was purchased from Qiagen GmbH.

Techniques: Knockdown, Expressing, Transfection, Western Blot, Binding Assay

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA‑4327 regulates TGF‑β1 stimulation of matrix metalloproteinase‑13 expression via CREB‑binding protein‑mediated Runx2 acetylation in human osteoblasts

doi: 10.3892/etm.2024.12770

Figure Lengend Snippet: Figure 2. Knockdown of CBP decreased TGF‑β1‑stimulated MMP‑13 expression in human osteoblasts. Scrambled siRNA or CBP siRNA (30 nM) was transiently transfected into MG‑63 cells for 24 h. Cells were treated with 5 ng/ml TGF‑β1 or left untreated for 8 h. Western blot analysis of the whole‑cell lysates was performed using antibodies against CBP, MMP‑13, or α‑Tubulin. α‑Tubulin expression was used for normalization. CBP, CREB‑binding protein; TGF‑β1, transforming growth factor‑β1; MMP‑13, matrix metalloproteinase‑13; si, small interfering.

Article Snippet: Scrambled control siRNA (cat. no. Sc‐37007) and CBP siRNA (cat. no. Sc‐29244) were purchased from Santa Cruz Biotechnology, Inc. miR‐4327 mimic (GeneGlobe ID: YM00470747) was purchased from Qiagen GmbH.

Techniques: Knockdown, Expressing, Transfection, Western Blot

Journal: Science Advances

Article Title: The regulatory enzymes and protein substrates for the lysine β-hydroxybutyrylation pathway

doi: 10.1126/sciadv.abe2771

Figure Lengend Snippet: ( A ) p300 knockdown by siRNA transfection impairs histone Kbhb in HCT116 cells. Histone Kbhb and Kac levels were analyzed by immunoblotting with indicated antibodies. NS, nonsilencing. ( B ) p300 knockout (KO) decreases histone Kbhb levels in HCT116 cells. The indicated histone PTMs were analyzed by immunoblotting with indicated antibodies. WT, wild type. ( C ) p300 inhibitor A485 reduces histone Kbhb and Kac levels dose-dependently in HCT116 cells. HCT116 cells were treated with A485 for 24 hours, and the Kbhb and Kac levels of histones were analyzed by immunoblotting with indicated antibodies.

Article Snippet: Anti-p300 antibody (sc-585) and CBP small interfering RNA (siRNA) (sc-29244) were purchased from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Knockdown, Transfection, Western Blot, Knock-Out